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What is the advantage of using a wet mount preparation instead of a dry mount in the study of living cells and can you explain why you could not use and oak leaf in this investigation?

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Answer:

In wet mount the specimen is suspended in liquid. This liquid can be salt water or water from the habitat of the organism and it is present between cover glass and slide.

When living cells are studied wet mount has following advantages over dry mount:

  • Image quality is improved due to refractive index of water
  • It is possible to observe real time cell functions like feeding and division.
  • Organisms are viewed in their natural form and colour as dry mount can fade or change the colour of the organism

Oak leaf can't be used in wet mount investigation because it is too thick. It blocks light coming from the stage and observer cant observe the specimen's details.

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