you have purified the enzyme enolase from both beef heart and e. coli. enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. you have carried out preliminary kinetic analysis and obtained the following data: Enzym source Km VmaxBeef Heart 1.9x10^-3 M 290 nmol/minE. Coli 24x10^-6 M 18 nmol/minYour boss ask you which enzyme is more efficient. What is your answer and why?

Respuesta :

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Answer:

The one from E coli

Explanation:

One way of determining catalytic efficiency is to calculate the ratio of Vmax to Km  

For beef heart,

[tex]\dfrac{V_{max}}{K_{m}} = \dfrac{290 \times 10^{-9}\text{ mol/min }}{1.9 \times 10^{-3}\text{ mol$\cdot$L}^{-1}} = 1.5 \times 10^{-5} \text{ L/min} = \mathbf{15 \, \mu \text{L/min}}[/tex]

For E coli ,

[tex]\dfrac{V_{max}}{K_{m}} = \dfrac{18 \times 10^{-9}\text{ mol/min }}{24 \times 10^{-6}\text{ mol$\cdot$L}^{-1}} = 7.5 \times 10^{-4} \text{ L/min} = 750 \, \mu\text{L/min}[/tex]

Thus, the enzyme from E. coli is 50 times as efficient as the one from beef hea rt.