Techniques that would not be suitable for determining the presence of a specific mRNA in a tissue sample are : RNA interference, RNA-seq, or microarray.
The most sensitive technique for detecting and quantifying mRNA is RT- PCR. Using RT-PCR, even small sample sizes can be used in the quantification of mRNA and the technique can do this using just a single cell.
A number of widely used procedures exist for detecting and determining the abundance of a particular mRNA in RNA sample. But four popular methods are: Northern blot analysis, nuclease protection assays (NPA), in situ hybridization and reverse transcription-polymerase chain reaction (RT-PCR).
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