Infrared spectroscopy using an infrared gas analyzer is used to measure the entering and exiting CO2 from the leaf chamber. The difference provides us the amount of CO2, from which we can compute the rate of photosynthesis.
A chemical reaction's volume of gas can be evaluated by collecting it in an inverted container filled with water. The gas pushes water out of the container, and the amount of liquid displaced is proportional to the amount of gas.
Enclosing a leaf in a closed, transparent container and monitoring the drop in carbon dioxide content as a function of time is how the photosynthetic rate of a leaf is assessed.
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