Consider that in a typical PCR you do not start with \"a single molecule\" of template DNA, but rather something in the range of 25 nanomoles of template DNA. What does this tell you about the potential of PCR to amplify DNA?

Respuesta :

Answer:

It definitely tells us that the PCR is a very powerful technique, because it allows us to obtain a great amount of copies from a specific DNA fragment or gene, starting from a really small sample of DNA.

Explanation:

The reason why de PCR technique works in that way is because the number of copies increases exponentially.

During the first PCR cycle, we have a single DNA molecule, which by denaturation separates into 2 DNA strands, then the primer annealing occurs so the DNA polymerase can start synthetizing the rest of the DNA. Therefore, this first cycle will produce 2^1=2 DNA fragments.

Then, in the second cycle we will start with 2 DNA molecules that will be separated again by denaturation, leaving 4 strands, this 4 strands will lead to the synthesis of 2^2=4 DNA fragments, in the third we will have 2^3=6 synthesized fragments, and so on.

This is why the PCR technique has so much potential to amplify DNA, giving also the possibility to control de number of DNA copies will get just by determining the number of cycles we will perform.

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