In the B-galactosidase assay, it is important to measure the density of E. coli cells because Select one:
a. the lac operon is induced only in a small percentage of cells in a culture.
b. the transformation efficiency of E. coli cells is low.
c. E. coli densities vary among cultures and with time, which will affect estimates of b-galactosidase production rates.
d. ONPG production by E. coli is a function of cell density.
e. we are measuring per cell cleavage of X-gal in relation to culture conditions and time.

Question

02-11-2019
Biology

contestada

In the B-galactosidase assay, it is important to measure the density of E. coli cells because Select one:
a. the lac operon is induced only in a small percentage of cells in a culture.
b. the transformation efficiency of E. coli cells is low.
c. E. coli densities vary among cultures and with time, which will affect estimates of b-galactosidase production rates.
d. ONPG production by E. coli is a function of cell density.
e. we are measuring per cell cleavage of X-gal in relation to culture conditions and time.

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podgorica podgorica · Answer 1 · 2019-11-05T04:33:27+01:00

Answer:

Option C, E. coli densities vary among cultures and with time, which will affect estimates of b-galactosidase production rates.

Explanation:

The activity of β-galactosidase depends on the optical density or the intensity of the color.

In regards to color, yellow color intensity signifies the qualitative parameter of the β-galactosidase activity while the readings obtained through colorimeter is quantitative parameter of the β-galactosidase activity. In the presence of lactose , the gene responsible for producing β-galactosidase in E. coli is either induced or switched off.

Hence , option D is correct