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Which of the following would be a reasonable experimental strategy to prevent translation of a particular prokaryotic mRNA without targeting the ribosome? Which of the following would be a reasonable experimental strategy to prevent translation of a particular prokaryotic mRNA without targeting the ribosome? Inhibit termination factors. Inhibit the sigma factor. Add suppressor tRNA molecules to the cell. Mutate or remove the Shine-Dalgarno sequence on the mRNA.

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Answer:

The correct answer is "Mutate or remove the Shine-Dalgarno sequence on the mRNA".

Explanation:

The Shine-Delgarno sequence is a fragment of bacterial and archaeal messenger RNA that helps to recruit the messenger RNA to the ribosome to initiate protein synthesis by translation. Mutating  or removing the Shine-Dalgarno sequence on the mRNA will prevent translation of a particular prokaryotic mRNA. Therefore, this is a reasonable experimental strategy to achieve this effect without targeting the ribosome, but the prokaryotic mRNA.

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