Respuesta :
1. Enzymes
2. Fingerprint
3. PCR
Explanation:
1. Restriction enzymes are those enzymes that cuts or cleave the DNA sequence at specific sites called recognition sites. The DNA pieces thus obtained is amplified and used for analysis and comparison. Restriction endonucleases cuts the phosphodiester bond of the DNA strand in the interior and Restriction exonucleases cuts at the ends.
2. DNA Fingerprint is the technique in which the DNA samples are run in the gel which separates the DNA strands based on its size which is then compared to the reference DNA sample. Therefore, DNA fingerprint is used in crime scene investigation. During the process of gel electrophoresis, the sample DNA is loaded into the gel with the help of a micropipette and then allowed to run in the presence of electric current. The DNA strands gets separated based on its size and charge.
3. Polymerase chain reaction (PCR) is the process of amplification of DNA. In crime scene investigation, the DNA sample of the suspect is amplified millions of times to evaluate and compare with the criminal's DNA sample using PCR. In the process of PCR, DNA template, DNA Polymerase enzyme (Taq polymerase), primers are required. Temperature plays an important role in the process of PCR.
