Using a spectrophotometer, and a cuvette with a path length of 1 cm you measure the absorbance (A275) of Guanosine to be 0.70. Calculate the concentration of guanosine in your sample

Respuesta :

Answer : The concentration of guanosine in your sample is, [tex]8.33\times 10^{-5}M[/tex]

Explanation :

Using Beer-Lambert's law :

[tex]A=\epsilon \times C\times l[/tex]

where,

A = absorbance of solution  = 0.70

C = concentration of solution = ?

l = path length = 1.00 cm

[tex]\epsilon[/tex] = molar absorptivity coefficient guanosine  = [tex]8400M^{-1}cm^{-1}[/tex]

Now put all the given values in the above formula, we get:

[tex]0.70=8400M^{-1}cm^{-1}\times C\times 1.00cm[/tex]

[tex]C=8.33\times 10^{-5}M[/tex]

Thus, the concentration of guanosine in your sample is, [tex]8.33\times 10^{-5}M[/tex]