Answer:
The substrate concentration reached Vmax
Explanation:
In this scenerio, further increase in the rate of reaction was prevented because the substrate concentration hit Vmax.
Beyond the Vmax, the substrate can no longer proceed.
- The substrate is the reactant on which the enzyme works on.
- Enzymes are natural organic chemical catalysts.
- Increasing the concentration of reactants is one of the known and proven ways to speed up the rate of reaction.
- Since we have limited amount of enzymes, once they all bound to the substrate, no further increase in the substrate will have an effect on the rate.
- At the optimum reaction point, all the available substrate will bond with the enzyme.
- Beyond this, the enzymes are saturated will not further any reaction.
