You use the Gram staining procedure to stain an L-form bacterium (a bacterium that lacks a cell wall). What color will the bacterium be after the staining procedure is finished

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Answer:

Pink

Explanation:

A microbiologist named Christian Gram invented the gram staining technique, which aims at using a colored substance (crystal violet in colour) called GRAM STAIN to identify bacteria cells. Using the gram staining procedure, bacteria cells are classified as either Gram-positive or Gram-negative.

Gram positive bacteria are those bacteria types that retain the crystal violet color of the gram stain due to the peptidoglycan content (high) of their cell wall. Hence, they appear PURPLE after the procedure. They don't get decolorized after the procedure.

On the other hand, Gram-negative bacteria does not retain the crystal violet color of the Gran stain (gets washed off) because they lack a peptidoglycan-containing cell wall. During the gram staining procedure, the gram-negative bacteria gets bleached using an alcohol, hence, is recolorized by another dye called SAFRANIN, which makes the gram-negative bacteria appear PINK.

The bacterium will attain pink color after staining is finished.

Gram stain

A test, which checks for bacteria at the location of a suspected infection like the lungs, throat, genitals, or in the wounds of the skin.

In the sample, when the stain combines with bacteria it will either turn pink or red or stay purple.

If the bacteria remains purple, they are considered as Gram positive.

In the L-form of bacteria, as they lack cell wall, they attain pink color, that is, they attain safranin dye.

In the L-form of bacteria, as they are devoid of cell wall, the decolorization of crystal violet takes place with ethanol, and then it attains pink color of safranin.

Thus, the L-form of bacteria will attain pink color.

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