Answer :
I think you forgot to attach the options along with the question. I am answering the question based on my research and knowledge. the two factors that affect an enzyme's operation the most are temperature and pH level. Enzymes function at at its best when pH is 7.4. I hope the answer helps you.
Considering the choices;
A. blood glucose level and pH
B. amount of light and temperature
C. amount of light and pressure
D. temperature and pH
Answer;
D. temperature and pH
The two factors affect an enzyme's operation the most is the temperature and pH.
Explanation;
Enzymes work best at optimum pH and temperature.
At low temperature for example the enzymes are inactivated while at high temperatures enzymes are denatured.
At optimal temperature and pH; the chemical reaction rate is optimal.
