Tetracycline is an antibiotic that blocks tRNA from entering the ribosome and associating with mRNA. If the tetracycline were added to the cytoplasm of a eukaryotic cell, which types of proteins would be made?
A. Only proteins that will be exported outside the cell
B. Only proteins that will be inserted into a membrane
C. Only proteins that function in the cytoplasm of the cell
D. No proteins will be made if tRNA cannot bind to mRNA

If the tetracycline is added to the cytoplasm of a eukaryotic cell, NO proteins will be made because tRNA cannot bind to mRNA (Option D is correct).

The transference RNAs (tRNAs) are fundamental during protein synthesis, (i.e., translation).

During translation, each triplet of nucleotides or 'codon' in the messenger RNA (mRNA) sequence binds by base complementarity to the anticodon of a given tRNA.

Each tRNA also contains a specific amino acid residue that is attached to its end, which is added to the nascent polypeptide chain in the ribosome.

Thus, the amino acids are specified by the codons of the mRNA sequence that bind to specific anticodons in tRNAs.

In conclusion, if the tetracycline is added to the cytoplasm of a eukaryotic cell, NO proteins will be made because tRNA cannot bind to mRNA (Option D is correct).

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