Roche/454 pyrosequencing , useful for high throughput sequencing techniques. This method utilizes pyrosequncing which allows for sequencing as the sequence read out can be achieved at the same time as the sequence is extended.
High- throughput screening is the use of automated equipment to rapidly test thousand to millions of samples for the biological activities at the model organism, cellular, pathway or molecular level.Many DNA samples can be sequenced simultaneously.
Sanger DNA sequencing also known as chain termination. This method is used only for the single DNA fragment at a time. Amplicon sequencing with NGS is efficient and cost effective . It is required a DNA polymerase , nucleotide , dideoxynucleotides and buffer.
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