Respuesta :
310 nm
310 nm wavelength of light is the best choice when attempting to quantitatively relate solution absorbance and concentration.
What does beer lambert law state?
The Beer-Lambert law states that "the quantity of light absorbed by a substance dissolved in a fully transmitting solvent is directly proportional to the concentration of the substance and the path length of the light through the solution."
- Here, the wavelength for absorption is 310 nm.
- We confirmed that the largest aggregation happens at this wavelength based on the preliminary experiments.
- Your spectrum measurements may be repeated at a very low concentration.
- From there, you may deduce the concentration and maximum absorbance at the relevant wavelength.
Applications of Beer-Lambert law:
- Beer-law Lambert's is used to analyze a combination using spectrophotometry without the sample needing to go through a lot of pre-processing.
- The measurement of bilirubin in blood plasma samples is one example.
- Since the molar absorbance is known, the spectrum of pure bilirubin is also known.
Limitations of Beer-Lambert law:
- light scattering as a result of sample-related particles.
- the sample's fluorescence or phosphorescence.
- refractive index modifications at high analyte concentrations.
- alterations in chemical equilibrium that occur as a result of concentration.
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