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You would have contamination of molecules that diffused outside of the sac, and your results would be inconclusive.The transformation performance could lower due to the fact with out warmth stunning the bacteria, the pGLO plasmid DNA could now no longer be capable of flow thru the mobileular membrane, and be picked up through the bacterial mobileular.
Yes, if a bacterium already contained the gene for ampicillin-resistance, it'd be feasible for it to develop at the LB plates containing ampicillin even though it had now no longer picked up the pGLO plasmid.
The mortar and pestle are used to open up the wheat germ cells through breaking down the mobileular walls. The cheek cells lack a mobileular wall so the mortar and pestle aren't required. To get the DNA into the bacteria, we should poke holes in them with the chemical calcium chloride (CaCl2).
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