Gel electrophoresis is normally set up with the negative electrode at the top of the gel and the positive electrode at the bottom of the gel. The DNA products are loaded at the top of the gel, and then a current is applied to separate them then DNA molecules will run toward the bottom.
The positive electrode is often placed at the bottom of the gel, and the negative electrode is typically placed at the top. At the top of the gel, the DNA products are added, and a current is then used to separate them. Due to the negative charge of DNA, when an electric current is supplied to the gel, DNA will move in the opposite direction, toward the positively charged electrode. The DNA fragments are ordered in order because shorter DNA strands pass through the gel more quickly than longer ones do.
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